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BB20144: Techniques in cell biology

Academic Year: 2018/9
Owning Department/School: Department of Biology & Biochemistry
Credits: 6      [equivalent to 12 CATS credits]
Notional Study Hours: 120
Level: Intermediate (FHEQ level 5)
Period:
Semester 1
Assessment Summary: EX 70%, PR 30%
Assessment Detail:
  • Practical (PR 30%)
  • Examination (EX 70%)
Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Requisites:
Before taking this module you must take BB10005
While taking this module you must take BB20024
Description: Aims:
To give an overview of some of the commonly used techniques in cell biology and to provide an understanding of how these techniques are being applied to address current cell biological problems.

Learning Outcomes:
After taking this course the student should be able to:
* Describe and explain the basic principles involved in each technique;
* Determine and explain when a technique should be used;
* Assess the strengths and weaknesses of each technique;
* Plan their time during the course of an experiment for maximal efficiency and optimal outcome.

Skills:
Learning and studying T/F/A, Written communication T/F/A, Laboratory skills T/F, Information technology T/F, Problem solving T/F, Information handling & retrieval T/F/A, Working independently T/F, Career preparation T/F.

Content:
Lectures will cover the basic principles underlying the techniques of light and electron microscopy including immunofluorescence and confocal microscopy, immunoblotting, immunoprecipitation, cell culture, transfection of mammalian cell lines including considerations of types of promoters and cell type specific expression, subcellular fractionation and metabolic labelling. The practical content will include projects focused on transfection of mammalian cell lines, immunofluroescence, immunoblottting, and subcellular fractionation.
Programme availability:

BB20144 is Compulsory on the following programmes:

Department of Biology & Biochemistry
  • USBB-AFB05 : BSc(Hons) Molecular and Cellular Biology (Year 2)
  • USBB-AKB08 : BSc(Hons) Molecular and Cellular Biology with Professional Placement (Year 2)

Notes:

  • This unit catalogue is applicable for the 2018/19 academic year only. Students continuing their studies into 2019/20 and beyond should not assume that this unit will be available in future years in the format displayed here for 2018/19.
  • Programmes and units are subject to change in accordance with normal University procedures.
  • Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.
  • Undergraduates: .
  • Postgraduates: .