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BB10186: Proteins: purification and characterisation

Academic Year: 2018/9
Owning Department/School: Department of Biology & Biochemistry
Credits: 6      [equivalent to 12 CATS credits]
Notional Study Hours: 120
Level: Certificate (FHEQ level 4)
Period:
Semester 2
Assessment Summary: EX 75%, MC 25%
Assessment Detail:
  • MCQ (MC 25%)
  • Exam (EX 75%)
Supplementary Assessment:
Like-for-like reassessment (where allowed by programme regulations)
Requisites: Before taking this module you must take BB10003 AND take CH10088
While taking this module you must take BB10121 AND take CH10087
Description: Aims:
To provide understanding of the principles and applications of various methods for the purification and characterisation of proteins.

Learning Outcomes:
After taking this unit the student should be able to:
* Describe common methods for purifying proteins and be able to design a simple protein purification scheme;
* Describe the use of different spectroscopic techniques to probe structural features of proteins;
* Describe physical techniques used to elucidate the function and oligomeric state of proteins.

Skills:
Learning and studying T/F/A, Written communication T/F/A, Numeracy & computation T/F/A, Laboratory skills T/F, Information technology T/F, Information handling & retrieval T/F/A, Working independently T/F.

Content:
Protein separation by column chromatography based on differences in molecular size, charge, hydrophobicity and specific affinity for ligands; electrophoretic methods for the purification and characterisation of proteins; use of techniques (such as fluorescence, UV/CD spectroscopy, dynamic light scattering, mass spectroscopy, ITC and SPR) to probe function and oligomeric state of proteins. 		Before taking this unit you must take BB10003 and take CH10088 and while taking this unit you must take BB10004 and take CH10087
Programme availability:

BB10186 is Compulsory on the following programmes:

Department of Biology & Biochemistry

Notes:

  • This unit catalogue is applicable for the 2018/19 academic year only. Students continuing their studies into 2019/20 and beyond should not assume that this unit will be available in future years in the format displayed here for 2018/19.
  • Programmes and units are subject to change in accordance with normal University procedures.
  • Availability of units will be subject to constraints such as staff availability, minimum and maximum group sizes, and timetabling factors as well as a student's ability to meet any pre-requisite rules.
  • Undergraduates: .
  • Postgraduates: .